Request pdf effect of chrysin on hepatoprotective and antioxidant status in dgalactosamineinduced hepatitis in rats chrysin is a natural, biologically active compound present in many plants. Liver damage was induced by intra peritoneal administration of 400 mgkg b wt dgalactosamine on the last day. Attenuation of oxidative stress and hepatotoxicity induced by d. Modification of the hepatotoxicity of dgalactosamine in the rat by cycloheximide. Protective effect of ultrasonicationprocessed ginseng berry extract. Hepatoprotective effect of pentoxifylline against dgalactosamine induced hepatotoxicity in rats. Effect of chrysin on hepatoprotective and antioxidant.
Acetylbergenin was synthesized from acetylation of bergenin, isolated from mallotus japonicus, to increase lipophilic and physiological activities. Suppressive effect of modified arabinoxylan from rice bran. Druginduced hepatotoxicity and hepatoprotective medicinal. Green tea extract supplement reduces dgalactosamine. The aim of this study was to assess d galactosamine d galnlipopolysaccharide lps induced hepatocyte apoptotic changes in mice and clarify the mechanisms involved in this process. Dgalactosamine is an amino sugar derivative of dgalactose item no. Modification of the hepatotoxicity of dgalactosamine in. Conclusion the eeib found to have significant hepatoprotective activity by dgaln induced. D galactosamine is a hepatotoxic agent, which induces diffuse injury of liver tissue followed by the regeneration process. We analyzed the molecular mechanism of galnlpsinduced apoptosis of hepatocytes and examined the therapeutic effects of etoposide on galn lpsinduced lethal liver injury. The selective sirt1 modulators whether activators stacs or inhibitors are being tried experimentally and clinically. The present study examined plasma lipid profiles in thirty patients suffered from acute viral hepatitis. Mar 25, 2009 oxidative stress and inflammation contributed to the propagation of acute liver injury ali. In colectomized rats, galn produced little hepatotoxicity, while it induced severe liver damage by simultaneous administration of lipopolysaccharide lps.
Normoactive smooth endoplasmic reticulum and modification by phenobarbital raymond s. Hepatotoxicity was induced in wistar rats by intraperitoneal injection of d galn 400 mgkg in saline in wistar rats. In this study, danhong injection dhi was investigated to evaluate the preventive and protective effect on ahf induced by lipopolysaccharide lps and dgalactosamine galn in mice. Effects of acetylbergenin against d galactosamineinduced. The appearance of d galactosamine induced hepatitis and generalized edema in. To isolate and identify the polyphenolic constituents of. Pdf hepatoprotective effect of phyllanthus niruri alkaloid fraction. The efficacy of dietary spirulina as an adjunct to. Dgalactosamine exerts its hepatotoxicity by causing intracellular deficiency of uridine metabolites, presumably in conjunction with other factors such as endotoxinaemia 66. Twenty four wistar strain albino rats were used for the investigations. The role of endotoxin in liver injury induced by d galactosamine galn was investigated in rats in vivo, or by using isolated rat hepatocytes in vitro.
Treatment of experimental animals with dgalactosamine galn lipopolysaccharide lps causes lethal liver injury that is characterized by apoptosis of the hepatocyte. Protective effects of luteolin against apoptotic liver. Dietary glycine or pretreatment with gdcl 3 prevented these effects. Treatment of experimental animals with d galactosamine galn lipopolysaccharide lps causes lethal liver injury that is characterized by apoptosis of the hepatocyte. Hepatobiliary clearance of labelled mebrofenin in normal and d galactosamine hcl induced hepatitis rats and the protective effect of turmeric extract. Impaired plasma lipid profiles in acute hepatitis lipids. Liver damage was induced by intra peritoneal administration of 400 mgkg b wt d galactosamine on the last day. Modulation of dgalactosaminelipopolysacharrideinduced. Selective uridine triphosphate deficiency induced by d. Drug induced hepatotoxicity is the leading cause of acute liver failure. D galactosamine is hepatotoxic and is used, alone or in combination with lps, as a model of liver failure in rodents.
Nature and mechanisms of hepatocyte apoptosis induced by d. Effect of lecithin on dgalactosamine induced hepatotoxicity. Curcumin attenuates dgalactosaminelipopolysaccharide. Hepatotoxic effect of dgalactosamine and protective role of. The protective effect of pinitol against dgalactosamine galninduced liver damage was examined. Xu yang1, masayoshi fujisawa1, teizo yoshimura1, toshiaki ohara1, miwa sato1, megumi mino1, thar htet san1, tong gao1, steven l.
Hepato protective activity of various extracts of indigofera. Liver damage was induced by intra peritoneal administration of 400 mgkg b wt dgalactosamine on the. Aug 23, 2011 twenty four wistar strain albino rats were used for the investigations. Pdf curcumin attenuates dgalactosaminelipopolysaccharide. Sabesin medical service, boston veterans administration hospital, and the department of medicine, boston university school of medicine. D galactosamine is observed only when a low dose of endotoxin is also administered,10 causing activation of kupffer cells and tumor necrosis factora tnfa release.
Pdf hepatoprotective activity of dypsis lutescens against d. Liver damage was induced by intra peritoneal administration of 400 mgkg b wt d galactosamine d galn. Dgalactosaminelipopolysaccharideinduced hepatotoxicity. The combination of lps and dgalactosamine galn is used experimentally to induce acute liver injury similar to fhf observed clinically. Glycine and uridine prevent dgalactosamine hepatotoxicity in the rat. Comprehensive analysis of differential gene expression pro. Original article picroside ii ameliorates acute liver. Hepatotoxicity was induced in wistar rats by intraperitoneal injection of dgaln 400 mgkg in saline in wistar rats. Hepatotoxic effect of dgalactosamine and protective role. The aim of this study was to assess dgalactosamine dgalnlipopolysaccharide lpsinduced hepatocyte apoptotic changes in mice and clarify the mechanisms involved in this process.
Dgalactosamine induces fulminant liver failure by intraperitoneal injection in doses of 200 mgkg 67. The aim of the present wok the hepatoprotective activity as well as the antioxidant potential of the ethyl acetate stem extract of m. In this study, danhong injection dhi was investigated to evaluate the preventive and protective effect on ahf induced by lipopolysaccharide lps and d galactosamine galn in mice. Lead optimization strategies to improve potency, efficacy, metabolic stability, and. D galactosamine induced utp deficiency is reversed completely within 90 min after uridine administration. Additionally, antisera to tumor necrosis factor tnf prevented hepatotoxicity caused byd.
Pdf hepatoprotective effect of pentoxifylline against d. Evaluation of druginduced tissue injury by measuring alanine. Effect of lecithin on d galactosamine induced hepatotoxicity. Liver injury induced by d galn has been used as the animal model for liver injury, since its morphological and pathophysiological characteristics are similar to those of human hepatits b. Luteolin and luteolin7oglucoside protect against acute liver injury through regulation of inflammatory mediators and antioxidative enzymes in galnlpsinduced hepatitic icr mice chung mu park, 1 and youngsun song 2. At the end of the study animals were sacrificed and liver enzyme levels, histopathology, mitochondrial integrity, expression of p53, bax. Plasma total triglycerides tg, total cholesterol, high density lipoprotein cholesterol hdlc, low density lipoprotein cholesterol ldl. Modification of the hepatotoxicity of d galactosamine in the rat by cycloheximide. Apr 24, 2014 mukherjee s, mahmoudi tm and mukherjee u. Thurman1,3 extrahepatic factors, such as increased gut permeability and bacteria from the gut, have been shown to play a role in. The animals were divided into four groups of six animals in each group.
Curcumin, a naturally occurring antioxidant, has various beneficial effects in the treatment of human diseases. Effects of dgalactosamineinduced acute liver injury on. The protective effect of pinitol against d galactosamine galn induced liver damage was examined. A class of triazole gpr142 agonists was discovered through a high throughput screen. Modification of the hepatotoxicity of dgalactosamine in the. Research article open access evaluation of hepatoprotective. Full text pdf 1764k abstracts references20 citedby5 the role of endotoxin in liver injury induced by dgalactosamine galn was investigated in rats in vivo, or by using isolated rat hepatocytes in vitro. Protective effect of danhong injection on acute hepatic. Apoptosis plays a role in the normal development of liver.
Hepatoprotective activity of lepidium sativum seeds against dgalactosamine lipopolysaccharide induced hepatotoxicity in animal model. The ali model was established by intraperitoneal i. Oxidative stress and inflammation contributed to the propagation of acute liver injury ali. Nacetyldgalactosamine galnac, an aminosugar, is a component of many olinked and nlinked glycan structures. In this study, the protective effects of luteolin 1, a major component of cirsium japonicum were examined against dgalactosamine galnlipopolysaccharide lpsinduced fulminant hepatic failure. Teas and other beverages suppress dgalactosamine induced liver injury in rats. The present study was aimed to evaluate the hepatoprotective activity of ethanolic extract of roots of tetracera akara root on d galactosamine induced hepatotoxicity in wistar rats. Ambrex was orally administered for a period of 7 days at dose levels of 250 and 500 mgkg b. Mice received an intraperitoneal injection of 1 25, 50, 100, and 200 mgkg1 1 h before treatment with galn 700 mgkg1lps 10. Sixth conference of the world day for research, riyadh, 2014. Liver transplant for viral hepatitis and fulminant hepatic failure. Spred2 deficiency exacerbates dgalactosaminelipopolysaccharide induced acute liver injury in mice via increased production of tnf xu. Warning this product is not for human or veterinary use. Fourth international conference on clinical nutrition and community nutrition, riyadh, 2008.
Our previous studies suggest silkworms can be used as model animals instead of mammals in pharmacologic studies to develop novel therapeutic medicines. Role of endotoxin in dgalactosamine induced hepatic injury. Dgalactosamine lipopolysaccharide induced acute liver injury in mice via increased production of tnf. Dgalactosamine is hepatotoxic and is used, alone or in combination with lps, as a model of liver failure in rodents.
The hepatoprotective effects of acetylbergenin were examined against dgalactosamine galn induced liver damage in rats, compared with that of bergenin reported previously. The lead compound 4 suffered from poor metabolic stability and poor solubility. Sirt1 modulators in experimentally induced liver injury. Acute hepatic failure ahf, which leads to an extremely high mortality rate, has become the focus of attention in clinic. Drugs that induce hepatotoxic effects in mammals were injected into the silkworm hemocoel, and. Pretreatment with eeib and silymarin for 21days protected the rat livers from d galactosamine induced hepatotoxicity.
Kemelo, institute of pharmacology, first faculty of medicine, charles university in prague, albertov 4, 128 00 prague 2, czech republic. Manual of histology, staining methods of armed forces, institute of pathology. Apoptotic, hepatoprotective and antioxidant potential of a triherbal formulation against dgalactosamine hepatotoxicity 2 introduction the liver is the primary site for drug metabolism therefore hepatotoxicity is one of the most frequently reported human adverse drug reactions. A combination of d galactosamine and lipopolysaccharide d galnlps downregulated sirt1 expression, while sirt1 activators, srt1720, resveratrol, and quercetin, upregulated sirt1 and alleviated d galnlps induced acute hepatotoxicity.
Drugs that induce hepatotoxic effects in mammals were injected into the silkworm. However, overactivation thereof may lead to hepatocellular damage. We discuss the modulation of sirt1 on cytoprotection or even cytotoxicity in the liver chemically. Antiinflammatory, antioxidant and antihepatotoxic effects of spirulina platensis against dgalactosamine induced hepatotoxicity in rats. Glycine and uridine prevent dgalactosamine hepatotoxicity in. Protective effects of luteolin against apoptotic liver damage. Patients blood samples were collected at both the debut and recovery of diseases.
However, little information regarding the protection it provides against acute liver. Evaluation of druginduced tissue injury by measuring. Experimental and molecular pathology 19, 168177 1973 dgalactosamine hepatotoxicity iii. The hepatoprotective effects of acetylbergenin were examined against d galactosamine galn induced liver damage in rats, compared with that of bergenin reported previously. Sabesin medical service, boston veterans administration hospital, and the department of medicine, boston university school of medicine, boston, massachusetts. Comprehensive analysis of differential gene expression.
Treatment with boswellia ovalifoliolata root extracts has protected liver from induced hepatotoxicity. Lecithin administration and dgalctosamine dgaln challenge. Male c57bl6 mice aged 68 weeks were randomized into three groups. However, treatment with silymarin and liv52 significantly decrease the biochemical markers, as compared to d galactosamine group. Sirt1 resveratrol ex527 dgalactosamine lipopolysaccharide hepatotoxicity corresponding author m. Liver damage was induced by intra peritoneal administration of 400 mgkg b wt dgalactosamine dgaln. Predicted data is generated using the us environmental protection agencys episuite. The hepatoprotective effects of acetylbergenin were examined against dgalactosamine galninduced liver damage in rats, compared with that of bergenin reported previously. Nacetyl d galactosamine galnac, an aminosugar, is a component of many olinked and nlinked glycan structures. Mechanistic study on flumequine hepatocarcinogenicity. Effect of chrysin on hepatoprotective and antioxidant status. Hepatoprotective effect of phyllanthus niruri alkaloid fraction in dgalactosamine induced hepatitis in rats. Hepatobiliary clearance of labelled mebrofenin in normal and dgalactosamine hclinduced hepatitis rats and the protective effect of turmeric extract.
Hepatoprotective activity of lepidium sativum seeds against d. Dgalactosamineinduced utp deficiency is reversed completely within 90 min after uridine administration. This was demonstrated by reducing the elevated levels of biochemical markers and additional histopathological observations have shown that there is an improvement in the structural design of liver due to induced hepatotoxicity. Lecithin administration and d galctosamine d galn challenge. When lipid emulsion was applied immediately after d galactosamine, the. The present study was aimed to evaluate the hepatoprotective activity of ethanolic extract of roots of tetracera akara root on dgalactosamine induced hepatotoxicity in wistar rats. However, treatment with silymarin and liv52 significantly decrease the biochemical markers, as compared to dgalactosamine group. Isoproterenol induced myocardial infarction in male wistar rats aristatile balakrishnan king saud university, saudi arabia title.
This article is directed at highlighting the involvement of the endogenous stress sensor sirt1 silent information regulator t1 as a possible factor involved in hepatoprotection. Model hepatotoxicants acetaminophen paracetamol acetaminophen also known as paracetamol or. The present study was undertaken to determine whether d galactosamine d galn induces ali via the mitochondrial apoptosis and proinflammatory cytokinesignaling pathways, and possible mechanisms by which green tea gt extract modulates the apoptotic and proinflammatory signaling in rat. Thirty sex and age matched normal subjects were included as controls. Discovery and optimization of a novel triazole series of. Original article picroside ii ameliorates acute liver injury. Pdf hepatoprotective activity of lepidium sativum seeds. Galactosamine definition of galactosamine by medical. D galactosamine induces fulminant liver failure by intraperitoneal injection in doses of 200 mgkg 67. Dgalactosamine galn is a hepatotoxin frequently used in the study of liver injury 1.
Forty male spraguedawley rats were divided into normal control, galn control, a. Effects of resveratrol and ex527 pretreatment in lipopolysaccharide induced hepatitis in d galactosamine sensitized rats d galnlps on plasma levels of alanine aminotransferase alt, aspartate aminotransferase ast a b and bilirubin c. Molecular mechanisms of dgalactosaminelipopolysaccharide. Protective effect of pinitol against d galactosamine. The appearance of dgalactosamineinduced hepatitis and generalized edema in. Hepg2 cell lines were used for in vitro study and dgalactosamine dgaln. Gpr142 has been identified as a potential glucosestimulated insulin secretion gsis target for the treatment of type 2 diabetes mellitus t2dm. Hepatocellular foci were observed in 2 out of 8 and 6 out of 7 animals in the flpb gal and flfl gal groups, respectively. The present study was undertaken to determine whether dgalactosamine dgaln induces ali via the mitochondrial apoptosis and proinflammatory cytokinesignaling pathways, and possible mechanisms by which green tea gt extract modulates the apoptotic and proinflammatory signaling.
D galactosamine exerts its hepatotoxicity by causing intracellular deficiency of uridine metabolites, presumably in conjunction with other factors such as endotoxinaemia 66. Liver injury markers exhibited an inverse relationship with sirt1 expression. Dgalactosamine hydrochloride cas 1772038 cayman chemical. Acetylbergenin was administered orally once daily for 7 days and then galn. Our data showed a high increase of serum aminotransferases after d galactosamine administration, which indicates a high extent of liver injury. Ameliorative effect of ethanolic extract of roots of. Glycine and uridine prevent dgalactosamine hepatotoxicity. This offers the possibility to inhibit utpdependent processes in vivo for periods corresponding to the time interval between galactosamine and uridine injection. Dgalactosamine induced hepatic damage was manifested by a significant increase in the activities of marker enzymes.
Lecithin 50 and 100 mgkg b wt was administered for 1 week by oral route. Hepatoprotective effects of il22 on fulminant hepatic failure induced by dgalactosamine and lipopolysaccharide in mice. Pretreatment with eeib and silymarin for 21days protected the rat livers from dgalactosamine induced hepatotoxicity. Green tea extract supplement reduces dgalactosamineinduced. We analyzed the molecular mechanism of galnlps induced apoptosis of hepatocytes and examined the therapeutic effects of etoposide on galn lps induced lethal liver injury. We examined the usefulness of the silkworm larvae bombyx mori as an animal model for evaluating tissue injury induced by various cytotoxic drugs. D galactosamine is an amino sugar derivative of d galactose item no. As udpgalnac, galnac is the intial olinked sugar to many serine and threonine residues in protein glycosylations. Protective effect of pinitol against d galactosamineinduced. Biochemical data exhibited significant hepatoprotective activity of methanol extract of portulaca oleracea at oral dose of 200 and 400 mgkg against dgalactosamine. A high dose of galn is known to induce apoptosis of hepatocytes in rat and mouse, which is evidenced by histochemical observations, dna laddering, and caspase 3 activation 24. In colectomized rats, galn produced little hepatotoxicity, while it induced severe liver damage by simultaneous administration of.
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